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  Indian J Med Microbiol
 

Figure 2: (a) Scaling of extracted tooth done. (b) Removal of cementum from outer surface. (c) Access opening and removal of pulpal tissue and irrigation with saline. (d) Hard tissue microtome. (e) Sectioning done with hard tissue microtome. (f) Sections placed in respective solutions. (g) Calcium level measured with atomic spectroscopy

Figure 2: (a) Scaling of extracted tooth done. (b) Removal of cementum from outer surface. (c) Access opening and removal of pulpal tissue and irrigation with saline. (d) Hard tissue microtome. (e) Sectioning done with hard tissue microtome. (f) Sections placed in respective solutions. (g) Calcium level measured with atomic spectroscopy