J Interdiscip Dentistry
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   2020| January-April  | Volume 10 | Issue 1  
    Online since April 30, 2020

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In vitro assessment of cytotoxicity and anti-inflammatory properties of shilajit nutraceutical: A preliminary study
S Sarah Victoria Ezhilarasi, Rajkumar Kothandaraman, Ravikumar Nesamani, Saravanakarthikeyan Balasubramanian, Sekar Mahalaxmi
January-April 2020, 10(1):24-28
Background: Shilajit is a nutraceutical exudate found mainly in the Indian Himalayas, which is formed for centuries by the gradual decomposition of certain plants due to microbial action. It has been effectively used as a potent and safe dietary supplement, restoring the energetic balance and potentially able to prevent several diseases. Hence, the aim of the present preliminary in vitro study is to assess the cytotoxicity of shilajit extract and to comparatively evaluate the anti-inflammatory effect of shilajit and diclofenac sodium, a commonly used drug. Materials and Methodology: Shilajit was commercially procured in pure powder form which was dissolved in 10 ml of methanol and boiled for 80 min, followed by centrifugation (2500 rpm) for 10 min. The supernatant fluid thus obtained was used as an experimental solution. 25-mg diclofenac sodium was used as a control drug. The cytotoxicity of shilajit extract was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, whereas the anti-inflammatory property of the test groups was comparatively evaluated using protein denaturation assay. Results: As the dilution of the extracts increased, the amount of cell viability was also increased, thereby showing that the diluted shilajit extract concentrations proved to be least cytotoxic. Different percentages of shilajit exhibited concentration-dependent inhibition of protein denaturation. Although shilajit extract exerted marginally better anti-inflammatory effect than diclofenac sodium, the effect being dose dependent; the protein inhibition values were not statistically significant (P > 0.05). Conclusion: Within the limitations of this in vitro preliminary study, it can be concluded that: (i) shilajit extract was found to be nontoxic when tested on L929 mouse fibroblast cell lines and (ii) the anti-inflammatory effect of shilajit was comparable to that of diclofenac sodium. Hence, this nutraceutical can be a viable alternative to conventional anti-inflammatory drugs in the field of medicine and dentistry.
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Oral and general health status of battery factory workers in Amman, Jordan: Cases presentations and review
Faiez Najeeb Hattab
January-April 2020, 10(1):9-16
Background: Workers in industrial battery factories frequently exposed to a hazardous working environment that causes adverse effects on their oral and general health. Aim: The aim of this study is to highlight oral and general health status among battery workers exposed to sulfuric acid-lead fumes and mists in the production site. Cases of deteriorated oral health presented. Subjects and Methods: The sample consisted of 24 participants working in the production site and 15 in the nonproduction line matched in age and employment period, acts as controls. Structural questionnaires on oral and general histories had been completed by interviews. Clinical examinations were carried out to assess dental erosion, oral hygiene, periodontal status using the appropriate indices. The data were statistically analyzed. Results: Questionnaires of workers in the production line revealed that more than two-thirds (67%–86%) of the workers had complaints of teeth sensitivity, dry mouth and nose, disturbed sense of taste, burning/itching eyes, and abdominal distress. They exhibited significantly higher scores of dental erosion, poor oral hygiene, and gingivitis compared with the control group (P < 0.05). The general health of the control group was much better than the workers in the production line.Conclusions: The presented cases revealed that unprotected workers in hazardous battery workplace suffer of serious oral and general health problems. These points the need of implementation efficient monitoring and control of acid vapor emission in the workplace coupled with appropriate oral and general health education and care.
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Laser therapy for myofascial pain dysfunction syndrome
Ankita Vikas Chitnis, Gaurang S Mistry, Padmapriya Puppala, Naina A Swarup
January-April 2020, 10(1):35-38
Myofascial pain dysfunction syndrome (MPDS) has been a very prevalent disorder with an enigmatic etiology and even difficult treatment protocol. Patients suffering from MPDS are difficult to diagnose and require exclusion of various symptoms and signs to formulate a treatment plan. In recent years, low-level laser therapy has been advocated as an effective way to counter and alleviate the resulting pain caused due to MPDS. This article is a case report on a successful reduction of pain in a patient diagnosed with the same.
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Determination of the antibacterial activity of atorvastatin against periodontal pathogens, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis: An in vitro study
Swetalin Das, Pushpa S Pudakalkatti, Ancia Vaz, Prabhdeep Kour, Sreeshma Padmanabhan
January-April 2020, 10(1):3-8
Aim: The aim of the present study was to determine the antimicrobial activity of pure atorvastatin drug against the primary periodontal pathogens. Materials and Methods: Both minimum inhibitory concentrations and minimum bactericidal concentrations were used to assess the antibacterial effect of atorvastatin against Actinobaillus actinomycetemcomitans and Prophyromonas gingivalis by serial dilution method and culture method, respectively. Results: In the present study, both A. actinomycetemcomitans and P. gingivalis were sensitive to pure atorvastatin drug. P. gingivalis was found to be more sensitive than A. actinomycetemcomitans in this study. Conclusions: Data suggest a potent antimicrobial activity of atorvastatin against both A. actinomycetemcomitans and P. gingivalis. Hence, atorvastatin can be prescribed as a dual action drug in patients suffering from both hyperlipidemia and periodontal disease.
  1,188 188 -
Identification of Dialister pneumosintes in healthy and chronic periodontitis patients with Type 2 diabetes mellitus and its correlation with the red complex bacteria
Pratima Oswal, Sandeep Katti, Vinayak Joshi, Hawaabi Shaikh
January-April 2020, 10(1):17-23
Aim: The aim of the study was to assess the presence of Dialister pneumosintes in healthy and chronic periodontitis (CP) patients with Type 2 diabetes mellitus and its association with the red complex bacteria. Materials and Methods: Depending on the inclusion and exclusion criteria, a total of 36 patients, aged 35–70 years, were allotted to two groups: periodontally healthy diabetic patients (18) and CP diabetic patients (18). Subgingival plaque samples were collected from the deepest site. Microbial assessment was done by subjecting the plaque samples to DNA isolation, and conventional polymerase chain reaction was performed for the identification of D. pneumosintes with specific primers. Results and Conclusion: D. pneumosintes was found greater in number in CP patients (50%) than in healthy controls (27.78%), but it was statistically insignificant (P = 0.17). Association of the presence of D. pneumosintes with Tannerella forsythia was 83.33%. It was found that there was a statistically significant association between D. pneumosintes and T. forsythia, with P = 0.047.
  1,108 145 -
Acumination of professionalism
S Mahalaxmi, KV Anitha, K Rajkumar
January-April 2020, 10(1):1-2
  966 162 -
Elastic deflection study of nickel-titanium orthodontic wires: 3-point bending test X clinical simulation device
Renata Sathler, Marcos Roberto de Freitas, Carlos Alberto Soufen, Marcelo Zanda, Thais Maria Freire Fernandes, Olga Benário Vieira Maranhão, Daniela Gamba Garib, Guilherme Janson
January-April 2020, 10(1):29-34
Introduction: The purpose of this study was to compare the most usual types of bending tests used to evaluate nickel-titanium orthodontic wires: the 3-point bending test and the clinical simulation device (CSD). Materials and Methods: Eleven groups of 0.014-inch nickel-titanium orthodontic wires of six different brands were tested. A 3342 Instron universal testing machine with a 10 N load cell was used, and the ISO 15.841 regulation was followed. T-tests were used to compare the evaluations. Results: At a deflection of 0.5 mm, the device released significantly lighter forces then the 3-point test. However, at deflections of 1; 2 and 3 mm, the device released significantly heavier forces. Conclusion: Because of the several differences between the tests and the inconsistency of the CSD, the 3-point bending test was elected the most reliable method to evaluate the elastic deflection of nickel-titanium orthodontic wires.
  843 94 -
A novel approach of rehabilitation of a microstomia patient with sectional hinged dentures
C S Arun Kumar, J Brintha Jei, K Murugesan, B Muthukumar
January-April 2020, 10(1):39-43
When the mouth opening is restricted, it can hinder the successful dental treatment. This limited mouth opening may be due to some surgical treatment, oral submucous fibrosis, temporomandibular joint disorder, burns, space infections, trauma, neoplasm, radiotherapy, and scleroderma. This case report describes the simplified prosthodontic management of a completely edentulous patient with limited mouth opening by providing sectional maxillary and mandibular dentures joined by hinges.
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I-implant notation system: A fundamental implant location system in clinical dentistry
Aruna Wimalarathna
January-April 2020, 10(1):44-47
This article is introducing a simple method to locate the implant positions in the dental arch during clinical examination and implant assessment. Basic signs of “I” and “i” which indicate a conventional and mini-dental implant, respectively. The use of the I-Implant Notation System (I-INS) is described by means of its application to common dental implant situations, especially when describe the location of the existing or planned implants locations in the mouth. In addition, the way of notation is blended with the commonly available notation systems. The examples of use of IINS with Universal numbering system, federation dentaire internationale Two-Digit Notation, and Palmer Notation are also illustrated. By using IINS, easily visualized and transcript the existing as well as future implants locations, especially during preliminary implant assessment while communicating among dental professionals and scientific writing.
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