J Interdiscip Dentistry
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ORIGINAL ARTICLE
Year : 2020  |  Volume : 10  |  Issue : 1  |  Page : 17-23

Identification of Dialister pneumosintes in healthy and chronic periodontitis patients with Type 2 diabetes mellitus and its correlation with the red complex bacteria


Maratha Mandal's N.G.H., Institute of Dental Sciences, Belagavi, Karnataka, India

Correspondence Address:
Dr. Pratima Oswal
Senior Lecturer, Tatyasaheb Kore Dental College and Research Centre, New Pargaon, Kolhapur, Maharashtra
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jid.jid_4_19

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Aim: The aim of the study was to assess the presence of Dialister pneumosintes in healthy and chronic periodontitis (CP) patients with Type 2 diabetes mellitus and its association with the red complex bacteria. Materials and Methods: Depending on the inclusion and exclusion criteria, a total of 36 patients, aged 35–70 years, were allotted to two groups: periodontally healthy diabetic patients (18) and CP diabetic patients (18). Subgingival plaque samples were collected from the deepest site. Microbial assessment was done by subjecting the plaque samples to DNA isolation, and conventional polymerase chain reaction was performed for the identification of D. pneumosintes with specific primers. Results and Conclusion: D. pneumosintes was found greater in number in CP patients (50%) than in healthy controls (27.78%), but it was statistically insignificant (P = 0.17). Association of the presence of D. pneumosintes with Tannerella forsythia was 83.33%. It was found that there was a statistically significant association between D. pneumosintes and T. forsythia, with P = 0.047.


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